How far from the road will they be? Depends on several factors....wind direction, time they spray, what exactly they use, etc. etc....But short answer, Yes, it will kill your bees.
Here in Louisiana there has been a recent coordinated and cooperative effort to work with mosquito control on this issue and our local parish (county) mosquito division has been great to work with. I'd suggest starting by having a conversation with them, either personally, or possibly inviting them to speak at your LBC to discuss options. If you can get them to spray after dark once your bees are in for the night, you "should" be fine. Another option is to ask them to not spray when they get near your house...depending on if you have neighbors and how close they are, this may or may not be a good option. Another option is to share your hive location and ask them if they must spray, to take wind direction into account and do the best they can to minimize your hives exposure.
As I said, our Mosquito control folks have been great to work with...I'd just start a dialogue on a polite note acknowledging the importance of the job they do, while expressing your concern for the health of your bees and wanting to know how you can best work with them for a win/win situation.