I hived a cut out. Missed the queen but got several swarm cells (I believe the colony had swarmed just before I did the cutout, so there was no queen present) About two weeks after hiving the bees, I checked to see if the new queen had emerged , mated, and begun to lay. I saw her, but she had a mite on her back. For some reason, I had this impulse to protect my queen by removing the mite with my finger. When I touched her, off the queen flew, never to return. The colony tried to raise another queen, but numbers got too low for them to sustain waiting for her to hatch and lay. Kicked myself pretty good for that one.