Those with actual experience please correct me if I'm wrong.., Seems like I remember reading your biggest drop will be the third day of the first treatment. Tomorrow's my third day. I can't say if my first treatment can be considered a full treatment. I tested it outside of the hive to get my vaporizing time down pat. Then I stuck it in the front entrance. Once the time was up I disconnected it and waited 10 minutes to pull it out of the hive. There was a bee right in the middle of where the O/A had been and it didn't look like all of it had vaporized, there was some black resin. Since then my brain has grew a little larger and my next treatment I will remove the oil pan tray and stick it in there. The screen bottom will keep the bees off of it.