I owned 20 over hives, queens originated from Australia. I almost got sting every times during inspection/harvesting, unless I heavily smoked them.
If you have some native colonies near you and you apiary gets blood from them, your strain does not stay in condition. I by a couple of queens from professionals and I take only one generation daugters.
If you nurse bees before night even calm becomes angry. - But I suppose you does not. I have just now 20 hives.
You should get new blood to you tiny apiary .