And a brush? Why, is your hair messy?
If you want the bees off a comb, give it a hard shake. Or you can hold it by one end, and give it a good thump or two on the landing board.
A bee brush is really good at making bees really irritable. If you do use a bee brush, DON'T brush with it. That just rolls the bees and makes them mad. Use the brush to flick the bees off the comb with small flicks.
Only on days that end in "y".

Seriously though, thanks a million for the heads up on the brush...I'll nix it, as I certainly don't want my girls to get irritated.