If you are absolutely certain they are queenless, you can get them to make a queen or re queen, your call.
I would give them a frame with eggs and young larvae not more than 3-4 days old and a frame or two of uncapped honey and see how they do.
If they are queenless, you'll have your answer within twenty four hours as they should begin to make queen cells.
However, if you do allow them the opportunity to make their own, you want as many bees in your boxes as possible for the endeavor.
You may need to condense your set up for maximum queen rearing results.
What's your set ups consist of?