A couple of years ago, my hive superseded their queen. I was unaware until I was working the hives and I heard that piping that the newly hatched queen makes. Found the hive she was in and just got to the right frame when she raced out. The old queen had never been as good as my others in their hives. I figured I'd let nature take its course, and put the frame back in the hive. A few days later, I observed the new queen in the hive, the old marked queen was nowhere to be found.
Seeing her race out of her cell was so exciting!!!
Good luck with your queening efforts-