Hive #1 was a nuc earlier this spring. The original queen was rejected (may have swarmed though). They reared a queen, she mated and started laying well.
There may have been a lose queen in the package so the one in the cage was killed.
They then started rearing emergencies cells couple of weeks later. They reared new cells, but after a couple of weeks of no eggs, I introduced a new queen. They accepted her and she started laying.
Then bees were either not happy with the queen or she was killed during inspection.
I checked one week later, could not find queen. Eggs present, but also 2 emergency cells, almost capped. Searched for queen, but didn't find her. I will look again, but pretty sure she is gone as I'm getting fairly good at finding her when needed.
They selected the best egg candidates as possible queens, if either turns out unsatisfactory the cycle will probably be repeated.
Any suggestions as to what may be going on? I will leave this hive alone and let them rear the emergency cells as they obviously need them. But wonder what could be going on. I'm not treating this hive differently from the others, location/conditions are the same. confusing!
Reinforce the population by putting in a frame of brood from one of your other hives and to see if they build more queen cells. More queen cells would be emergency and indicate they were not happy with the emergency queens already started.
BTW, when buying queens, find out from the queen supplier if he uses chemicals (Apistan, Mite-away, etc) in his operation. If he does you can probably bet that any queen you get from him will be rejected or superceded quickly. There is growing evidence that mite control chemicals, along with the host of others the bees are subjected to, has a detrimental effect on queens.